INHIBITORY EFFECT AND ENZYMOLYSIS KINETICS OF LENTINAN ON Α-GLUCOSIDASE

Meifu Wu, Hongli Zhou

ABSTRACT:Objective: To study the inhibitory activity and enzymolysis kinetics of lentinan on α-glucosidase. Methods: The enzyme-agent screening model was used to investigate the optimal conditions of α-glucosidase action. In vitro enzyme kinetics was studied by changing the substrate concentration, reaction time, pH conditions and temperature. Results: The optimal reaction conditions of α-glucosidase were reaction time 120min, reaction temperature 50°C, buffer pH 6.0, concentration of substrate PNPG 0.1089 mol/L. Conclusion: 1g/L concentration of lentinan has good inhibitory activity against α-glucosidase, and the inhibitory effect of lentinan is competitive inhibition.

Keywords:Lentinan, α-glucosidase, enzymolysis kinetics.