PURIFICATION OF ANTHOCYANINS FROM FRUIT OF ARONI AMELANOCARPA

Song Jiangang, Zhong Fangli, Wang Xiaolin and Feng Yangyang

ABSTRACT:Five different macroporous resins were evaluated for the adsorption properties of the anthocyanins extracted from the Aroni amelanocarpa fruit. The effects of initial anthocyanin concentration, amount of elution solvent, elution velocity and ethanol concentration were studied by the dynamic adsorption/desorption method. Results showed LSA-10 possessed the best purification capability. The optimum adsorption conditions were 120 mL of 55.77 µg/mL sample, 1.5 mL/min of flow rate. The elution solvent was 120 mL of 50 % ethanol at velocity of 1.5 mL/min. After being processed by LSA-10, the purity of anthocyanin in the crude extracts increased from 0.24 % to 1.96 %.

Keywords:Aronia melanocarpa, Anthocyanin, Macroporous adsorption resin, Purification.